Engineer the Appropriate Environment for Nitrogen-Fixation within the Nodule

The symbiotic organ provides a location suitable for both multiplying beneficial bacteria and exchanging nutrients with the host plant.

The Symbiotic Organ Provides a Location

The nodule provides a suitable environment for nitrogen fixation, involving the delivery of sufficient levels of essential nutrients to the bacteria and regulating the free-flow of oxygen to maximise the functionality of the nitrogenase enzyme. A number of the proteins and processes involved in providing this environment are already known. In the ENSA project we consider this step as the final component to engineer and until we have tackled bacterial infection and nodule organogenesis, we will not begin engineering the appropriate environment for nitrogen fixation.